Peptide Synthesis
Pepscan Presto uses state of the art solid-phase resin based FMOC chemistry for the synthesis of custom peptides in quantities ranging from milligram to gram scale. The peptides are synthesized using modern synthesizers with controlled conditions during synthesis and optimized pre-activation chemistry and double couplings to ensure optimal reaction conditions for the highest quality of peptides. Depending on your requirements, the peptides can be modified at any position in the sequence with labels or fluorescent groups, or labeled or capped at the C- and N-terminus.
High throughput analysis of the synthesized peptides is performed by using Ultra Performance Liquid Chromatography (UPLC) in combination with Mass Spectrometry in order to assure quality and identity of the peptides.
Peptides are purified by HPLC, monitored by UV or Mass Spectrometry. Our highly automated HPLC/MS preparative system allows for the efficient purification of large numbers of peptides. For complex purifications we use a variety of columns and chromatography conditions to obtain the requested purities. Depending on your needs the peptides can be delivered in different purities (>70%, >80%, >90%, or >95%)
Custom peptides at Pepscan Presto:
- From milligram to gram scale
- Highest quality through resin-based FMOC chemistry
- Delivery freeze-dried in vials, 96-well plates or 384-well plates
- Incorporation of unnatural amino acids
- Incorporation or attachment of a wide range of tags and labels
- Quality control using MS or LC/MS
- Fully automated, Mass-Spec driven HPLC purification
- Aliquoting services