Fluorescently labeled peptides
Fluorescently labeled peptides are frequently used for in vivo biomedical imaging, protein binding and localization studies. Fluorochrome-conjugated peptides can be visualized by fluorescence microscopy or other fluorescence visualization techniques. Pepscan applies a wide variety of fluorescent labels. These labels can be incorporated at any desired position within your peptide. Most frequently, they are incorporated at the N-terminal end, or at the side chain of a cysteine (Cys) or lysine (Lys) residue, e.g. at the C-terminal end.
Examples of frequently used fluorescent dyes are:
Excitation / Emission wavelengths
Selected references for Fluorescently Labeled Peptides
Worch et al: Biochim. Biophys. Acta (2017) – PMID 27825831. Three conserved C-terminal residues of influenza fusion peptide alter its behavior at the membrane interface.
Christakos et al: Virology (2016) – PMID 26655242. PhiXing-it, displaying foreign peptides on bacteriophage ΦX174.
Lüchtenborg et al: Biosci. Rep (2015) – PMID 26487707. Mode of interaction of the Gαo subunit of heterotrimeric G proteins with the GoLoco1 motif of Drosophila Pins is determined by guanine