Fluorescently labeled peptides

Fluorescently labeled peptides are frequently used for in vivo biomedical imaging, protein binding and localization studies. Fluorochrome-conjugated peptides can be visualized by fluorescence microscopy or other fluorescence visualization techniques. Pepscan applies a wide variety of fluorescent labels. These labels can be incorporated at any desired position within your peptide. Most frequently, they are incorporated at the N-terminal end, or at the side chain of a cysteine (Cys) or lysine (Lys) residue, e.g. at the C-terminal end.

Examples of frequently used fluorescent dyes are:

Short name

Full name

Structure

Excitation / Emission wavelengths

FITC

Fluoresceinisothiocyanate

FITC

490 nm
520 nm

Abz

Anthranilyl, 2-Aminobenzoyl

abz

320 nm
420 nm

5-FAM

5-Carboxyfluorescein

fam

492 nm
517 nm

6-FAM

6-Carboxyfluorescein

fam

492 nm
517 nm

TAMRA

Carboxytetramethyl rhodamine

tamra

543 nm
572 nm

Dansyl

5-(Dimethylamino) naphthalene-1-sulfonyl

dansyl

342 nm
562 nm

EDANS

5-[(2-Aminoethyl)amino] naphthalene-1-sulfonic acid

edans

340 nm
490 nm

Cy3

1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E)-3-(1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium chloride

cy33

550 nm
570 nm

Cy5 peptide

1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dienyl)-3H-indolium chloride

cy51

650 nm
670 nm

Mca

7-Methoxycoumarinyl-4-acetyl

mca

325 nm
392 nm

Selected references for Fluorescently Labeled Peptides

Worch et al: Biochim. Biophys. Acta (2017) – PMID 27825831. Three conserved C-terminal residues of influenza fusion peptide alter its behavior at the membrane interface.

Christakos et al: Virology (2016) – PMID 26655242. PhiXing-it, displaying foreign peptides on bacteriophage ΦX174.

Lüchtenborg et al:  Biosci. Rep (2015) – PMID 26487707. Mode of interaction of the Gαo subunit of heterotrimeric G proteins with the GoLoco1 motif of Drosophila Pins is determined by guanine.

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