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Fluorescently labeled peptides

Fluorescently labeled peptides are frequently used for in vivo biomedical imaging, protein binding and localization studies. Fluorescent dye labeled peptides can be visualized by fluorescence microscopy or other fluorescence visualization techniques. Pepscan makes high quality fluorescent peptides via custom synthesis with wide variety of fluorescent labels, such as FITC, TAMRA, Alexa, Cy3 and many more. These labels can be incorporated at any desired position within your peptide. Most frequently, they are incorporated at the N-terminal end, or at the side chain of a cysteine (Cys) or lysine (Lys) residue, e.g. at the C-terminal end.

Examples of frequently used fluorescent dyes are

Short name

Full name

Structure

Excitation / Emission wavelength

FITC

Fluoresceinisothiocyanate

FITC [1]

490 nm
520 nm

Abz

Anthranilyl, 2-Aminobenzoyl

abz [2]

320 nm
420 nm

5-FAM

5-Carboxyfluorescein

fam [3]

492 nm
517 nm

6-FAM

6-Carboxyfluorescein

fam [3]

492 nm
517 nm

TAMRA

Carboxytetramethyl rhodamine

tamra [4]

543 nm
572 nm

Dansyl

5-(Dimethylamino) naphthalene-1-sulfonyl

dansyl [5]

342 nm
562 nm

EDANS

5-[(2-Aminoethyl)amino] naphthalene-1-sulfonic acid

edans [6]

340 nm
490 nm

Cy3

1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E)-3-(1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium chloride

cy33 [7]

550 nm
570 nm

Cy5

1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dienyl)-3H-indolium chloride

cy51 [8]

650 nm
670 nm

Mca

7-Methoxycoumarinyl-4-acetyl

mca [9]

325 nm
392 nm

Selected references for Fluorescently Labeled Peptides

Worch et al: Biochim. Biophys. Acta (2017) – PMID 27825831. Three conserved C-terminal residues of influenza fusion peptide alter its behavior at the membrane interface.

Christakos et al: Virology (2016) – PMID 26655242. PhiXing-it, displaying foreign peptides on bacteriophage ΦX174.

Lüchtenborg et al:  Biosci. Rep (2015) – PMID 26487707. Mode of interaction of the Gαo subunit of heterotrimeric G proteins with the GoLoco1 motif of Drosophila Pins is determined by guanine.