Fast, panoramic snapshots of the activity of 100’s of kinases in cells and tissues
Protein kinases exert their functions in complex networks comprised of interconnected signal transduction pathways. Abnormalities in these pathways are implicated in the etiology of numerous diseases. PepChip Kinase peptide microarrays provide you information about hundreds of kinase activities in cells or tissues.
Samples are assayed on over 1,000 different substrates in triplicate. The isotopic detection used is highly sensitive and is not hampered by substrate bias. These profiling products open up a range of new applications in drug development and signal transduction research by improving reducing development time, profiling enzyme specificity and providing new information on enzyme and enzyme-inhibitor specificity.
Purified protein kinases
PepChip profiling is highly suited to characterize individual protein kinases. This allows interrogation of over 1,000 different substrates in a single experiment in order to obtain insight in the substrate preference of novel kinases.
For known and novel kinases, a substrate profile is a good starting point to select optimal substrates for assay development, taking into account sensitivity as well as selectivity. Thus, PepChip Kinase is ideally suited for:
- Characterization of novel kinases
- Kinase assay development
- Multiplex assay design
Kinase profiling in cells and tissues
PepChip profiling is an excellent tool for comparing kinase networks in diseased versus healthy tissues, stimulated versus control and many more. PepChip profiling is compatible with a multitude of cell and tissue samples, including cultured cells, fresh white blood cells, liver, kidney, biopsies, resection tissue and so on. Comparative kinase profiling can be applied for:
- Comparison of healthy versus diseased tissue
- Effects of growth factors, hormones, inhibitors
- Stem cell differentiation time series
- Small biopsies and large samples
- Cultured and fresh cells
For detailed information and specifications of the kinase profiling technology, click here.
Selected References for PepChip Kinase Arrays
Bhatt et al: Proc. Natl. Acad. Sci. USA (2016) – PMID: 27342859. A viral kinase mimics S6 kinase to enhance cell proliferation.
De Bourayne et al: J. Leukoc. Biol (2016) – PMID: 27707883. Protein kinase CK2 controls T-cell polarization through dendritic cell activation in response to contact sensitizers.
Garaud et al: Cell. Mol. Immunol (2016) – PMID: 27499044. CD5 expression promotes IL-10 production through activation of the MAPK/Erk pathway and upregulation of TRPC1 channels in B lymphocytes.
Perner et al: Anticancer Res (2016) – PMID: 27919943. Kinomics Screening Identifies Aberrant Phosphorylation of CDC25C in FLT3-ITD-positive AML.
Scholma et al: Sci Rep (2016) – PMID: 27225531. Improved intra-array and interarray normalization of peptide microarray phosphorylation for phosphorylome and kinome profiling by rational selection of relevant spots.
Van der Sligte et al: Proteomics (2015) – PMID: 25422122. Kinase activity profiling reveals active signal transduction pathways in pediatric acute lymphoblastic leukemia: a new approach for target discovery.