PepChip Protease Profiling
Ready-to-use peptide arrays for rapid and comprehensive protease characterization
Proteases constitute one of the primary targets groups in drug discovery. The functional and regulatory capacities of proteases vary greatly and include, but are not limited to, blood clotting, extracellular matrix degradation, activation of apoptosis, protein degradation, and viral protein processing. Examples of approved drugs are ACE-inhibitors for hypertension, HIV-1 protease inhibitors for HIV infections, proteasome inhibitors for Multiple Myeloma and Dipeptidyl aminopeptidase 4 (DPP-4) inhibitors for diabetes. For assay design and characterization of novel proteases, insight in substrate preference and reaction conditions is important. Protease substrate microarrays can provide this information, outperforming classic individual assays in terms of speed per datapoint and scope. The PepChip Protease array displays as many as 1,024 individual biotinylated protease substrates in triplicate on a glass slide. The specific microarray allows investigation of protease substrate preference and identification of substrates. Uncleaved substrates are detected before and after digestion using commercially available dyes e.g. fluorescein-labeled streptavidin. PepChip Protease arrays are available as consumable and can be used in standard microarray scanners accepting 25 × 75 mm slides.
|Quantification||PepChip Protease Grid.zip|
|Substrate-list||PepChip Protease Map.zip|