6-FAM
Fluorescently labeled peptides
Fluorescently labeled peptides are frequently used for in vivo biomedical imaging, protein binding and localization studies. Fluorochrome-conjugated peptides can be visualized by fluorescence microscopy or other fluorescence visualization techniques. Pepscan applies a wide variety of fluorescent labels. These labels can be incorporated at any desired position within your peptide. Most frequently, they are incorporated at the N-terminal end, or at the side chain of a cysteine (Cys) or lysine (Lys) residue, e.g. at the C-terminal end.
Examples of frequently used fluorescent dyes are:
Short name
Full name
Structure
Excitation / Emission wavelengths
FITC
Fluoresceinisothiocyanate
490 nm
520 nm
Abz
Anthranilyl, 2-Aminobenzoyl
320 nm
420 nm
5-FAM
5-Carboxyfluorescein
492 nm
517 nm
6-Carboxyfluorescein
492 nm
517 nm
TAMRA
Carboxytetramethyl rhodamine
543 nm
572 nm
Dansyl
5-(Dimethylamino) naphthalene-1-sulfonyl
342 nm
562 nm
EDANS
5-[(2-Aminoethyl)amino] naphthalene-1-sulfonic acid
340 nm
490 nm
Cy3
1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E)-3-(1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium chloride
550 nm
570 nm
Cy5
1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dienyl)-3H-indolium chloride
650 nm
670 nm
Mca
7-Methoxycoumarinyl-4-acetyl
325 nm
392 nm
Selected references for Fluorescently Labeled Peptides
Worch et al: Biochim. Biophys. Acta (2017) – PMID 27825831. Three conserved C-terminal residues of influenza fusion peptide alter its behavior at the membrane interface.
Christakos et al: Virology (2016) – PMID 26655242. PhiXing-it, displaying foreign peptides on bacteriophage ΦX174.
Lüchtenborg et al: Biosci. Rep (2015) – PMID 26487707. Mode of interaction of the Gαo subunit of heterotrimeric G proteins with the GoLoco1 motif of Drosophila Pins is determined by guanine.
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