Fluorescently labeled peptides
Fluorescently labeled peptides are frequently used for in vivo biomedical imaging, protein binding and localization studies. Fluorescent dye labeled peptides can be visualized by fluorescence microscopy or other fluorescence visualization techniques. Pepscan makes high quality fluorescent peptides via custom synthesis with wide variety of fluorescent labels, such as FITC, TAMRA, Alexa, Cy3 and many more. These labels can be incorporated at any desired position within your peptide. Most frequently, they are incorporated at the N-terminal end, or at the side chain of a cysteine (Cys) or lysine (Lys) residue, e.g. at the C-terminal end.
Examples of frequently used fluorescent dyes are
Short name
Full name
Structure
Excitation / Emission wavelength
FITC
Fluoresceinisothiocyanate
490 nm
520 nm
Abz
Anthranilyl, 2-Aminobenzoyl
320 nm
420 nm
5-FAM
5-Carboxyfluorescein
492 nm
517 nm
6-FAM
6-Carboxyfluorescein
492 nm
517 nm
TAMRA
Carboxytetramethyl rhodamine
543 nm
572 nm
Dansyl
5-(Dimethylamino) naphthalene-1-sulfonyl
342 nm
562 nm
EDANS
5-[(2-Aminoethyl)amino] naphthalene-1-sulfonic acid
340 nm
490 nm
Cy3
1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E)-3-(1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium chloride
550 nm
570 nm
Cy5
1-(5-carboxypentyl)-3,3-dimethyl-2-((1E,3E,5E)-5-(1,3,3-trimethylindolin-2-ylidene)penta-1,3-dienyl)-3H-indolium chloride
650 nm
670 nm
Mca
7-Methoxycoumarinyl-4-acetyl
325 nm
392 nm
Selected references for Fluorescently Labeled Peptides
Worch et al: Biochim. Biophys. Acta (2017) – PMID 27825831. Three conserved C-terminal residues of influenza fusion peptide alter its behavior at the membrane interface.
Christakos et al: Virology (2016) – PMID 26655242. PhiXing-it, displaying foreign peptides on bacteriophage ΦX174.
Lüchtenborg et al: Biosci. Rep (2015) – PMID 26487707. Mode of interaction of the Gαo subunit of heterotrimeric G proteins with the GoLoco1 motif of Drosophila Pins is determined by guanine.
