Non-naturals replacement scans and validation
A positional scanning library is a key technique for sequence optimization. By sequentially substituting selected amino acid residues for all other natural L-amino acids, residues that are crucial for activity can be identified, and substitutions that display improved activity can also be found.
Positional scanning library design usually begins by focusing on the naturally occurring L-amino acids. However, should you want to tap into the non-natural amino acid space, non-natural amino acids can also be included. There are over 400 non-natural amino acids that are commercially available. Pepscan has 200 of these in stock which can be readily implemented in your library design. Another 200 non-natural amino acids are in our database and can included on request.
Combinatorial positional scanning libraries are an extension of the positional scanning libraries, systematically varying by two or more positions at a time. These libraries allow for the identification of more complex effects of multiple positions on the biological activity of a peptide sequence.