T-cell Epitope Mapping

Adaptive immunity keeps us alive thanks to its unique ability to recognize and kill pathogens. Adaptive immunity is orchestrated by B-cells and T-cells carrying antigen-specific surface receptors, which recognize linear and conformational elements (epitopes) of the pathogen’s structure. Specificity and selectivity of antigen – receptor interactions control humoral and cell-mediated immunity. Identifying T- and B-cell epitopes opens up possibilities for:

  • Immune profiling
  • Development of future diagnostics
  • Vaccine design
  • Discovery of anti-drug responses
  • Design of allergen immunotherapies

T-cell ELISpot

Pepscan has already developed a proprietary array-based platform reliably profiling epitopes of antibodies and antibody-like molecules (incl. IgG, IgE, IgM etc.). Our epitope mapping service has now been expanded with T-cell epitope mapping. The main challenge in T-cell epitope mapping is the low frequency of antigen-specific T-cells.

This challenge can be easily addressed epitope mapping with ELISpot, as it rapidly quantifies T-cell responses at the single-cell resolution. Certainly, the quality of peptide libraries used to reconstruct epitopes is the key to success in ELISpot assays. Here, the long-standing expertise of Pepscan’s scientists in peptide synthesis, accompanied by in-depth knowledge in adaptive immunity and epitope mapping, significantly strengthens our T-cell mapping service allowing to deliver high-quality results to our customers.

Left panel: Schematic illustration of a typical ELISpot experiment. A typical ELISpot experiment includes 5 main steps. Firstly, a capture antibody is immobilized onto the membrane’s surface. Secondly, peptides followed by PBMCs are added to the wells. Upon stimulation, cytokines are released and captured by the immobilized capture antibody. The results are visualized with a complimentary antibody and an appropriate HRP conjugate.

Right panel: Depiction of ELISpot results in positive (top) and negative (bottom) control wells. The bar graphs show higher spot counts with the active peptide when compared to spot counts with two different low-frequency peptides. ***p<0.001, data are mean + s.e.m., unpaired student t-test. Data represent ELISpot analyses from n=3 wells.

Why T-cell epitope mapping with Pepscan?

  • High-quality peptide libraries
  • High throughput method
  • Sensitive and accurate detection
  • Fast turnaround time
  • > 20 years in-house expertise in epitope mapping
  • Integration with antibody epitope mapping
  • Scientific support