|Peptide synthesis||FMOC chemistry. Maximum peptide length over 40 residues. All amino acids including D-amino acids and non-natural amino acids.|
|Capacity||50.000 peptides per run with custom high-througput parallel synthesis robots.|
|Peptide library format||Proprietary ‘Minicard’ format with solid phase-bound peptide constructs in 455 microwells. Surface chemistry: proprietary polymeric graft optimized for low non-specific binding and high peptide construct loading.|
|Combinatorial library complexity||Matrix analysis e.g. 50 x 50 = 2.500 double loop T3 CLIPS™. All matrix combinations within 40-mers possible. All overlapping single loops usually 15 – 20-mers. All overlapping peptides of a protein usually 15 – 20-mers. Full positional scan libraries of all epitopes.|
|Spatial construct complexity||Single loops on T2 CLIPS.
Double loop combinations on T3 or 2 x T2 CLIPS
Sheet-like T2 CLIPS. Helix-like T2 CLIPS.
All loop structures with 2-6 cysteines and 1 or 2 CLIPS.
|Peptide library reusability||At least 20 times but up to 100 depending on the samples. Library storage and re-use up to years.|
|Binding detection||Binding of the antibodies to the CLIPS peptides is determined in an ELISA. The resulting color in each well is quantified with a CDD camera.|
|Binding detection sensitivity||Optimized for epitope mapping of even low affinity binding antibodies (down to Kd=10-3M)|
|Required material and information||20 μg antibody or 20 μl polyclonal serum. Sequence of target protein in FASTA format or UniProt ID.|
|Project run-through time||Priority 1.5 months. Standard up to 3 months.|
|Reporting||Binding values of all peptides are quantified and stored in the PepLab™ database. A full report is provided including details on binding and specificity for each residue optimized for registration regulatory and/or IP purposes. Full support is offered for IP generation and publishing.|